Department of Cancer Biology; Department of Cell Biology
Animals; *Antigens, CD; Ascitic Fluid; Female; G(M1) Ganglioside; Galactosylceramides; Glycolipids; Glycosphingolipids; Inflammation; *Lactosylceramides; Leukemia, Experimental; *Macrophage Activation; Macrophages; Membrane Lipids; Mice; Mice, Inbred C57BL
We have characterized the major glycolipid constituents of the mouse peritoneal macrophage, and have demonstrated that alterations in the amount and in the accessibility of specific glycolipid species to galactose oxidase/NaB3H4 labeling, an indicator of glycolipid surface exposure, occur in response to inflammation and as a consequence of activation to a tumoricidal state. The key findings are: (a) Asialo GM1, a major neutral glycolipid constituent of all macrophage populations examined, is accessible to galactose oxidase/NaB3H4 labeling on the surface of TG-elicited and BCG-activated macrophages but not on resident macrophages; (b) GM1 is the predominant ganglioside constituent of the mouse macrophage. Resident macrophages contain two distinct GM1 species, as determined by cholera toxin binding, while TG-elicited and BCG-activated macrophages contain an additional GM1 species. Differences in the relative amounts of these GM1 species, as well as in their accessibility to galactose oxidase/NaB3H4 labeling, exist among the macrophage populations. These observations suggest that both a chemical and spatial reorganization of surface glycolipids occurs in response to inflammation and tumoricidal activation.
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Citation: J Exp Med. 1984 Oct 1;160(4):1114-25. Link to article on publisher's website