Role of SeqA and Dam in Escherichia coli gene expression: a global/microarray analysis
Department of Biochemistry and Molecular Pharmacology
Medical Subject Headings
Bacterial Outer Membrane Proteins; Chromosomes, Bacterial; DNA Methylation; DNA, Bacterial; DNA-Binding Proteins; Escherichia coli; Escherichia coli Proteins; Gene Expression Regulation, Bacterial; *Genes, Bacterial; Oligonucleotide Array Sequence Analysis; SOS Response (Genetics); Site-Specific DNA-Methyltransferase (Adenine-Specific); Transcription Factors
High-density oligonucleotide arrays were used to monitor global transcription patterns in Escherichia coli with various levels of Dam and SeqA proteins. Cells lacking Dam methyltransferase showed a modest increase in transcription of the genes belonging to the SOS regulon. Bacteria devoid of the SeqA protein, which preferentially binds hemimethylated DNA, were found to have a transcriptional profile almost identical to WT bacteria overexpressing Dam methyltransferase. The latter two strains differed from WT in two ways. First, the origin proximal genes were transcribed with increased frequency due to increased gene dosage. Second, chromosomal domains of high transcriptional activity alternate with regions of low activity, and our results indicate that the activity in each domain is modulated in the same way by SeqA deficiency or Dam overproduction. We suggest that the methylation status of the cell is an important factor in forming and/or maintaining chromosome structure.
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Citation: Proc Natl Acad Sci U S A. 2003 Apr 15;100(8):4672-7. Epub 2003 Apr 7. Link to article on publisher's site
Lobner-Olesen, Anders; Marinus, Martin G.; and Hansen, Flemming G., "Role of SeqA and Dam in Escherichia coli gene expression: a global/microarray analysis" (2003). Biochemistry and Molecular Pharmacology Publications and Presentations. Paper 51.