Quantitation of Dam methyltransferase in Escherichia coli
Department of Biochemistry and Molecular Pharmacology
Medical Subject Headings
Blotting, Western; Escherichia coli; Escherichia coli Proteins; Immunoenzyme Techniques; Methyltransferases; *Site-Specific DNA-Methyltransferase (Adenine-Specific)
An antiserum against Escherichia coli Dam methyltransferase has been developed in rabbits and employed to detect and quantitate the enzyme in immunoblots. A wild-type, rapidly growing E. coli cell (doubling time = 30 min) was found to contain about 130 molecules of Dam methyltransferase.
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Citation: J Bacteriol. 1992 Mar;174(5):1682-5.
Boye, Erik; Marinus, Martin G.; and Lobner-Olesen, Anders, "Quantitation of Dam methyltransferase in Escherichia coli" (1992). Biochemistry and Molecular Pharmacology Publications and Presentations. Paper 39.