Title

Quantitation of Dam methyltransferase in Escherichia coli

UMMS Affiliation

Department of Biochemistry and Molecular Pharmacology

Date

3-1-1992

Document Type

Article

Medical Subject Headings

Blotting, Western; Escherichia coli; Escherichia coli Proteins; Immunoenzyme Techniques; Methyltransferases; *Site-Specific DNA-Methyltransferase (Adenine-Specific)

Abstract

An antiserum against Escherichia coli Dam methyltransferase has been developed in rabbits and employed to detect and quantitate the enzyme in immunoblots. A wild-type, rapidly growing E. coli cell (doubling time = 30 min) was found to contain about 130 molecules of Dam methyltransferase.

Rights and Permissions

Citation: J Bacteriol. 1992 Mar;174(5):1682-5.

Related Resources

Link to Article in PubMed

PubMed ID

1537808