Identification of the gene (aroK) encoding shikimic acid kinase I of Escherichia coli
UMass Chan Affiliations
Department of Biochemistry and Molecular PharmacologyDocument Type
Journal ArticlePublication Date
1992-01-01Keywords
Amino Acid SequenceBase Sequence
Chromosomes, Bacterial
Cloning, Molecular
DNA Transposable Elements
Escherichia coli
*Genes, Bacterial
Lac Operon
Molecular Sequence Data
Mutation
Open Reading Frames
Phosphotransferases
*Phosphotransferases (Alcohol Group Acceptor)
Transcription, Genetic
Bacteria
Biochemistry, Biophysics, and Structural Biology
Enzymes and Coenzymes
Genetic Phenomena
Pharmacology, Toxicology and Environmental Health
Metadata
Show full item recordAbstract
DNA sequence analysis has revealed that an unidentified open reading frame (ufr1) is present immediately preceding the aroB gene of Escherichia coli. The predicted protein product of urf1 contains a consensus ATP-binding-site sequence and shows 34% amino acid homology to shikimate kinase II in a 97-amino-acid region. Inactivation of urf1 by insertion of an antibiotic resistance gene had a polar effect on aroB, indicating that these two genes constitute a transcriptional unit. The auxotrophic requirements of a strain mutant for both urf1 and aroL (encoding shikimate kinase II) are consistent with shikimate kinase deficiency. We propose that urf1 encodes shikimate kinase I and that it be designated aroK.Source
J Bacteriol. 1992 Jan;174(2):525-9.
DOI
10.1128/jb.174.2.525-529.1992Permanent Link to this Item
http://hdl.handle.net/20.500.14038/26098PubMed ID
1309529Related Resources
ae974a485f413a2113503eed53cd6c53
10.1128/jb.174.2.525-529.1992