Direct role of the Escherichia coli Dam DNA methyltransferase in methylation-directed mismatch repair
Department of Biochemistry and Molecular Pharmacology
Medical Subject Headings
Cloning, Molecular; *DNA Repair; DNA, Bacterial; Escherichia coli; Methylation; Methyltransferases; Mutation; Phenotype; Site-Specific DNA-Methyltransferase (Adenine-Specific); Substrate Specificity; T-Phages
The T4 dam+ gene has been cloned (S. L. Schlagman and S. Hattman, Gene 22:139-156, 1983) and transferred into an Escherichia coli dam-host. In this host, the T4 Dam DNA methyltransferase methylates mainly, if not exclusively, the sequence 5'-GATC-3'; this sequence specificity is the same as that of the E. coli Dam enzyme. Expression of the cloned T4 dam+ gene suppresses almost all the phenotypic traits associated with E. coli dam mutants, with the exception of hypermutability. In wild-type hosts, 20- to 500-fold overproduction of the E. coli Dam methylase by plasmids containing the cloned E. coli dam+ gene results in a hypermutability phenotype (G.E. Herman and P. Modrich, J. Bacteriol. 145:644-646, 1981; M.G. Marinus, A. Poteete, and J.A. Arraj, Gene 28:123-125, 1984). In contrast, the same high level of T4 Dam methylase activity, produced by plasmids containing the cloned T4 dam+ gene, does not result in hypermutability. To account for these results we propose that the E. coli Dam methylase may be directly involved in the process of methylation-instructed mismatch repair and that the T4 Dam methylase is unable to substitute for the E. coli enzyme.
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Citation: J Bacteriol. 1986 Mar;165(3):896-900.
Schlagman, Samuel L.; Hattman, Stanley; and Marinus, Martin G., "Direct role of the Escherichia coli Dam DNA methyltransferase in methylation-directed mismatch repair" (1986). Biochemistry and Molecular Pharmacology Publications and Presentations. Paper 27.