Phenotypic reversal in dam mutants of Escherichia coli K-12 by a recombinant plasmid containing the dam+ gene
Department of Biochemistry and Molecular Pharmacology
Medical Subject Headings
Cloning, Molecular; DNA (Cytosine-5-)-Methyltransferase; DNA, Recombinant; Escherichia coli; *Genes, Bacterial; Methyltransferases; Mutation; Phenotype; Plasmids; Site-Specific DNA-Methyltransferase (Adenine-Specific); *Transformation, Bacterial
A recombinant plasmid, pMQ3, carrying the dam gene of Escherichia coli K-12, was constructed and transformed into dam+ and dam- strains. Both dam- and dam+ strains containing pMQ3 showed a wild phenotype for all traits, including mutation rate, except for a 10-fold increase in DNA adenine methylase activity.
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Citation: J Bacteriol. 1983 Jan;153(1):562-5.
Arraj, Judy A. and Marinus, Martin G., "Phenotypic reversal in dam mutants of Escherichia coli K-12 by a recombinant plasmid containing the dam+ gene" (1983). Biochemistry and Molecular Pharmacology Publications and Presentations. Paper 18.