Title

Phenotypic reversal in dam mutants of Escherichia coli K-12 by a recombinant plasmid containing the dam+ gene

UMMS Affiliation

Department of Biochemistry and Molecular Pharmacology

Date

1-1-1983

Document Type

Article

Medical Subject Headings

Cloning, Molecular; DNA (Cytosine-5-)-Methyltransferase; DNA, Recombinant; Escherichia coli; *Genes, Bacterial; Methyltransferases; Mutation; Phenotype; Plasmids; Site-Specific DNA-Methyltransferase (Adenine-Specific); *Transformation, Bacterial

Abstract

A recombinant plasmid, pMQ3, carrying the dam gene of Escherichia coli K-12, was constructed and transformed into dam+ and dam- strains. Both dam- and dam+ strains containing pMQ3 showed a wild phenotype for all traits, including mutation rate, except for a 10-fold increase in DNA adenine methylase activity.

Rights and Permissions

Citation: J Bacteriol. 1983 Jan;153(1):562-5.

Related Resources

Link to Article in PubMed

PubMed ID

6336742