Direct observation of single protein molecules in aqueous solution
Department of Biochemistry and Molecular Pharmacology; RNA Therapeutics Institute
Medical Subject Headings
Carbocyanines; Diffusion; Microscopy, Fluorescence; Microscopy, Video; Sensitivity and Specificity; Solutions; Streptavidin; Time Factors; Water
Single molecule tracking (SMT) by fluorescence video microscopy has matured to be a well established method in the last few years. In this report we demonstrate the imaging and tracking of single protein molecules in a phosphate/Hepes buffer with high sensitivity at frame rates of 350 Hz.
Grunwald, David; Hoekstra, Andreas; Dange, Thomas; Buschmann, Volker; and Kubitscheck, Ulrich, "Direct observation of single protein molecules in aqueous solution" (2006). Biochemistry and Molecular Pharmacology Publications and Presentations. Paper 176.