Isolation and characterization of Dam+ revertants and suppressor mutations that modify secondary phenotypes of dam-3 strains of Escherichia coli K-12
Department of Biochemistry and Molecular Pharmacology
Medical Subject Headings
Adenine; Crosses, Genetic; DNA (Cytosine-5-)-Methyltransferase; Escherichia coli; *Genes; Methyltransferases; Mutation; Phenotype; *Recombination, Genetic; *Suppression, Genetic
Bacteria mutant in the dam (DNA adenine methylation) gene and in either recA or recB or recC genes are inviable (Virm- phenotype). From crosses between dam-3 bacteria and recA1 or recB21 recC22 strains, Vrm+ recombinants were recovered. Among these recombinants, Dam+ revertants were present which did not show the phenotypes normally associated with dam-3 bacteria. Three classes of indirectly suppressed strains (dam-3 genotype) were also recovered which showed alterations in the secondary phenotypes normally associated with dam-3 bacteria. These strains contained a second unlinked mutation in either mutL or mutS or sin. In addition, mutation in either sbcA or sbcB supresses the Vrm- phenotype of dam-3 recB21 recC22 strains.
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Citation: Mol Gen Genet. 1980;178(2):309-15.
McGraw, Bonnie R. and Marinus, Martin G., "Isolation and characterization of Dam+ revertants and suppressor mutations that modify secondary phenotypes of dam-3 strains of Escherichia coli K-12" (1980). Biochemistry and Molecular Pharmacology Publications and Presentations. Paper 16.