Title

Mass spectrometry tools for analysis of intermolecular interactions

UMMS Affiliation

Department of Pediatrics; Department of Biochemistry and Molecular Pharmacology

Date

7-24-2012

Document Type

Book Chapter

Medical Subject Headings

Mass Spectrometry; Protein Binding; Binding Sites

Abstract

The small quantities of protein required for mass spectrometry (MS) make it a powerful tool to detect binding (protein-protein, protein-small molecule, etc.) of proteins that are difficult to express in large quantities, as is the case for many intrinsically disordered proteins. Chemical cross-linking, proteolysis, and MS analysis, combined, are a powerful tool for the identification of binding domains. Here, we present a traditional approach to determine protein-protein interaction binding sites using heavy water ((18)O) as a label. This technique is relatively inexpensive and can be performed on any mass spectrometer without specialized software.

Comments

Citation: Methods Mol Biol. 2012;896:387-98. Link to article on publisher's site

Related Resources

Link to Article in PubMed