Hyper-recombination in dam mutants of Escherichia coli K-12
Department of Biochemistry and Molecular Pharmacology
Medical Subject Headings
Chromosome Mapping; Chromosomes, Bacterial; DNA (Cytosine-5-)-Methyltransferase; Escherichia coli; Genotype; *Mutation; *Recombination, Genetic; Species Specificity
F-prime heterogenotes of dam-3 bacteria segregate F-prime homogenotes at a frequency 30-200 times higher than the isogenic dam+ strain. A hyperrecombination mutant which shows increased recombination between chromosomal duplications was characterized as a dam mutant. The dam-3 allele causes a reduction in linkage of proximal unselected markers in transconjugants and increases the recombination frequency between a pair of closely linked markers. It is concluded that dam mutations confer a hyperrecombination phenotype to the cell.
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Citation: Mol Gen Genet. 1976 Dec 22;149(3):273-7.
Marinus, Martin G. and Konrad, E. Bruce, "Hyper-recombination in dam mutants of Escherichia coli K-12" (1976). Biochemistry and Molecular Pharmacology Publications and Presentations. 13.