Title

Removal of PEG and salt from recombinant human tumor necrosis factor-alpha(rhTNF-alpha) with serial operation of gel filtration chromatography

UMMS Affiliation

Department of Biochemistry and Molecular Pharmacology

Date

11-13-2001

Document Type

Article

Medical Subject Headings

Chromatography, Gel; Polyethylene Glycols; Recombinant Proteins; Sodium Chloride; Tumor Necrosis Factor-alpha

Abstract

A serial gel filtration operation with the combination of G25 and G100 in one column was developed to remove salt and PEG from upper phase rich in recombinant human tumor necrosis factor alpha after simultaneous cell disruption and two-phase aqueous extraction. Buffer exchanging and primary purification were also achieved at same time. The purification factor of 4.4 was obtained with an activity recovery of 97% by one single step of serial operation.

Rights and Permissions

Citation: Sheng Wu Gong Cheng Xue Bao. 2001 Jul;17(4):471-3.

Comments

Article is written in Chinese

Related Resources

Link to Article in PubMed

PubMed ID

11702713