Title

The RNase PARN-1 Trims piRNA 3' Ends to Promote Transcriptome Surveillance in C. elegans

UMMS Affiliation

RNA Therapeutics Institute; Program in Bioinformatics and Integrative Biology; Program in Molecular Medicine

Date

2-25-2016

Document Type

Article

Disciplines

Biochemistry, Biophysics, and Structural Biology | Bioinformatics | Cell Biology | Computational Biology | Integrative Biology | Systems Biology

Abstract

Piwi-interacting RNAs (piRNAs) engage Piwi proteins to suppress transposons and are essential for fertility in diverse organisms. An interesting feature of piRNAs is that, while piRNA lengths are stereotypical within a species, they can differ widely between species. For example, piRNAs are mainly 29 and 30 nucleotides in humans, 24 to 30 nucleotides in D. melanogaster, and uniformly 21 nucleotides in C. elegans. However, how piRNA length is determined and whether length impacts function remains unknown. Here, we show that C. elegans deficient for PARN-1, a conserved RNase, accumulate untrimmed piRNAs with 3' extensions. Surprisingly, these longer piRNAs are stable and associate with the Piwi protein PRG-1 but fail to robustly recruit downstream silencing factors. Our findings identify PARN-1 as a key regulator of piRNA length in C. elegans and suggest that length is regulated to promote efficient transcriptome surveillance.

Rights and Permissions

Citation: Cell. 2016 Feb 25;164(5):974-84. doi: 10.1016/j.cell.2016.02.008. Link to article on publisher's site

Related Resources

Link to Article in PubMed

PubMed ID

26919432