Simultaneous generation of many RNA-seq libraries in a single reaction

UMMS Affiliation

Program in Bioinformatics and Integrative Biology; Bioinformatics Core



Document Type


Medical Subject Headings

Bacteria; *Base Sequence; Gene Expression Profiling; *Gene Library; Sequence Analysis, RNA; Time Factors


Biochemistry, Biophysics, and Structural Biology | Bioinformatics | Computational Biology | Integrative Biology | Systems Biology


Although RNA-seq is a powerful tool, the considerable time and cost associated with library construction has limited its utilization for various applications. RNAtag-Seq, an approach to generate multiple RNA-seq libraries in a single reaction, lowers time and cost per sample, and it produces data on prokaryotic and eukaryotic samples that are comparable to those generated by traditional strand-specific RNA-seq approaches.

Rights and Permissions

Citation: Nat Methods. 2015 Apr;12(4):323-5. doi: 10.1038/nmeth.3313. Epub 2015 Mar 2. Link to article on publisher's site

Related Resources

Link to Article in PubMed

PubMed ID