Title

The HP1 homolog rhino anchors a nuclear complex that suppresses piRNA precursor splicing

UMMS Affiliation

Program in Molecular Medicine; Program in Bioinformatics and Integrative Biology; Department of Biochemistry and Molecular Pharmacology; RNA Therapeutics Institute

Date

6-5-2014

Document Type

Article

Medical Subject Headings

Animals; Chromosomal Proteins, Non-Histone; DEAD-box RNA Helicases; Drosophila Proteins; Drosophila melanogaster; Female; Ovary; *RNA Splicing; RNA, Small Interfering; RNA-Binding Proteins; SOXD Transcription Factors

Disciplines

Biochemistry, Biophysics, and Structural Biology | Bioinformatics | Computational Biology | Integrative Biology | Systems Biology

Abstract

piRNAs guide an adaptive genome defense system that silences transposons during germline development. The Drosophila HP1 homolog Rhino is required for germline piRNA production. We show that Rhino binds specifically to the heterochromatic clusters that produce piRNA precursors, and that binding directly correlates with piRNA production. Rhino colocalizes to germline nuclear foci with Rai1/DXO-related protein Cuff and the DEAD box protein UAP56, which are also required for germline piRNA production. RNA sequencing indicates that most cluster transcripts are not spliced and that rhino, cuff, and uap56 mutations increase expression of spliced cluster transcripts over 100-fold. LacI::Rhino fusion protein binding suppresses splicing of a reporter transgene and is sufficient to trigger piRNA production from a trans combination of sense and antisense reporters. We therefore propose that Rhino anchors a nuclear complex that suppresses cluster transcript splicing and speculate that stalled splicing differentiates piRNA precursors from mRNAs.

Rights and Permissions

Citation: Cell. 2014 Jun 5;157(6):1353-63. doi: 10.1016/j.cell.2014.04.030. Link to article on publisher's site

Comments

Co-authors Zhao Zhang and Fan Zhang are both doctoral students in the Interdisciplinary Graduate Program in the Graduate School of Biomedical Sciences (GSBS) at UMass Medical School.

Related Resources

Link to Article in PubMed