Optical recognition of converted DNA nucleotides for single-molecule DNA sequencing using nanopore arrays
Department of Biochemistry and Molecular Pharmacology; Program in Bioinformatics and Integrative Biology
Medical Subject Headings
DNA, Circular; *Nanotechnology; Sequence Analysis, DNA
Bioinformatics | Computational Biology | Molecular Biology | Nanoscience and Nanotechnology
We demonstrate the feasibility of a nanopore based single-molecule DNA sequencing method, which employs multicolor readout. Target DNA is converted according to a binary code, which is recognized by molecular beacons with two types of fluorophores. Solid-state nanopores are then used to sequentially strip off the beacons, leading to a series of detectable photon bursts, at high speed. We show that signals from multiple nanopores can be detected simultaneously, allowing straightforward parallelization to large nanopore arrays.
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Citation: Nano Lett. 2010 Jun 9;10(6):2237-44. doi: 10.1021/nl1012147. Link to article on publisher's site
McNally, Ben; Singer, Alon; Yu, Zhiliang; Sun, Yingjie; Weng, Zhiping; and Meller, Amit, "Optical recognition of converted DNA nucleotides for single-molecule DNA sequencing using nanopore arrays" (2010). Program in Bioinformatics and Integrative Biology Publications and Presentations. Paper 36.