Title

Sequence features that drive human promoter function and tissue specificity

UMMS Affiliation

Department of Biochemistry and Molecular Pharmacology; Program in Bioinformatics and Integrative Biology

Date

7-2010

Document Type

Article

Medical Subject Headings

Base Composition; Base Sequence; Binding Sites; Cell Line; Computational Biology; Epigenesis, Genetic; Gene Expression; Hep G2 Cells; Hepatocyte Nuclear Factor 4; Humans; Organ Specificity; Promoter Regions, Genetic; Protein Binding; Transcription, Genetic; Transfection

Disciplines

Bioinformatics | Computational Biology | Genetics and Genomics | Molecular Biology | Systems Biology

Abstract

Promoters are important regulatory elements that contain the necessary sequence features for cells to initiate transcription. To functionally characterize a large set of human promoters, we measured the transcriptional activities of 4575 putative promoters across eight cell lines using transient transfection reporter assays. In parallel, we measured gene expression in the same cell lines and observed a significant correlation between promoter activity and endogenous gene expression (r = 0.43). As transient transfection assays directly measure the promoting effect of a defined fragment of DNA sequence, decoupled from epigenetic, chromatin, or long-range regulatory effects, we sought to predict whether a promoter was active using sequence features alone. CG dinucleotide content was highly predictive of ubiquitous promoter activity, necessitating the separation of promoters into two groups: high CG promoters, mostly ubiquitously active, and low CG promoters, mostly cell line-specific. Computational models trained on the binding potential of transcriptional factor (TF) binding motifs could predict promoter activities in both high and low CG groups: average area under the receiver operating characteristic curve (AUC) of the models was 91% and exceeded the AUC of CG content by an average of 23%. Known relationships, for example, between HNF4A and hepatocytes, were recapitulated in the corresponding cell lines, in this case the liver-derived cell line HepG2. Half of the associations between tissue-specific TFs and cell line-specific promoters were new. Our study underscores the importance of collecting functional information from complementary assays and conditions to understand biology in a systematic framework.

Rights and Permissions

Citation: Genome Res. 2010 Jul;20(7):890-8. doi: 10.1101/gr.100370.109. Epub 2010 May 25. Link to article on publisher's site

Related Resources

Link to Article in PubMed