UMMS Affiliation

RNA Therapeutics Institute; Program in Bioinformatics and Integrative Biology; Wellstone Muscular Dystrophy Program, Department of Neurology; Program in Molecular Medicine; Department of Biochemistry and Molecular Pharmacology; Department of Molecular, Cell and Cancer Biology

Date

6-14-2017

Document Type

Article

Disciplines

Biochemistry, Biophysics, and Structural Biology | Bioinformatics | Computational Biology | Integrative Biology | Systems Biology

Abstract

CRISPR is widely used to disrupt gene function by inducing small insertions and deletions. Here, we show that some single-guide RNAs (sgRNAs) can induce exon skipping or large genomic deletions that delete exons. For example, CRISPR-mediated editing of beta-catenin exon 3, which encodes an autoinhibitory domain, induces partial skipping of the in-frame exon and nuclear accumulation of beta-catenin. A single sgRNA can induce small insertions or deletions that partially alter splicing or unexpected larger deletions that remove exons. Exon skipping adds to the unexpected outcomes that must be accounted for, and perhaps taken advantage of, in CRISPR experiments.

Rights and Permissions

Copyright © The Author(s). 2017. Citation: Genome Biol. 2017 Jun 14;18(1):108. doi: 10.1186/s13059-017-1237-8. Link to article on publisher's site

Comments

Full author list omitted for brevity. For full list of authors see article.

Related Resources

Link to Article in PubMed

Keywords

CRISPR/Cas9 genome editing, single-guide RNAs, sgRNAs, exon skipping

PubMed ID

28615073

Creative Commons License

Creative Commons Attribution 4.0 License
This work is licensed under a Creative Commons Attribution 4.0 License.

 
 

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