Title

Identifying Regions Enriched in a ChIP-seq Data Set (Peak Finding)

UMMS Affiliation

Program in Bioinformatics and Integrative Biology; Department of Biochemistry and Molecular Pharmacology

Publication Date

2-1-2017

Document Type

Article

Disciplines

Bioinformatics | Computational Biology

Abstract

This protocol demonstrates how to use MACS software to call peaks in a ChIP-seq data set. MACS is one of the most widely used peak-calling programs. The protocol describes how to run MACS in a UNIX environment and on Galaxy and how to extract the sequences of +/-50 bp around the summits of the 500 MACS peaks with the most significant P values.

Rights and Permissions

Citation: Cold Spring Harb Protoc. 2017 Feb 1;2017(2):pdb.prot093187. doi: 10.1101/pdb.prot093187. Link to article on publisher's site

Related Resources

Link to Article in PubMed

Journal/Book/Conference Title

Cold Spring Harbor protocols

PubMed ID

27574193