Title

Saitohin, which is nested in the tau locus and confers allele-specific susceptibility to several neurodegenerative diseases, interacts with peroxiredoxin 6

UMMS Affiliation

Department of Cell Biology

Date

9-28-2005

Document Type

Article

Medical Subject Headings

Alleles; Animals; Base Sequence; COS Cells; Cell Line; Cercopithecus aethiops; DNA; Haplotypes; Heredodegenerative Disorders, Nervous; System; Humans; Peroxidases; Peroxiredoxin VI; Peroxiredoxins; Pick Disease of the Brain; RNA Splicing; Recombinant Fusion Proteins; Two-Hybrid System Techniques; tau Proteins

Disciplines

Cell Biology

Abstract

Saitohin is a gene unique to humans and their closest relatives, the function of which is not yet known. Saitohin contains a single polymorphism (Q7R), and its Q and R alleles belong to the H1 and H2 tau haplotype, respectively. The Saitohin Q allele confers susceptibility to several neurodegenerative diseases. To get a handle on Saitohin function, we used it as a bait in a yeast two-hybrid screen. By this assay and subsequent co-immunoprecipitation and glutathione S-transferase pull-down assays, we discovered and confirmed that Saitohin interacts with peroxiredoxin 6, a unique member of that family that is bifunctional and the levels of which increase in Pick disease. The strength of the interaction appeared to be allele-specific, giving the first distinction between the two forms of Saitohin.

Rights and Permissions

Citation: J Biol Chem. 2005 Nov 25;280(47):39268-72. Epub 2005 Sep 26. Link to article on publisher's site

Related Resources

Link to Article in PubMed