Saitohin, which is nested in the tau locus and confers allele-specific susceptibility to several neurodegenerative diseases, interacts with peroxiredoxin 6
Department of Cell Biology
Medical Subject Headings
Alleles; Animals; Base Sequence; COS Cells; Cell Line; Cercopithecus aethiops; DNA; Haplotypes; Heredodegenerative Disorders, Nervous; System; Humans; Peroxidases; Peroxiredoxin VI; Peroxiredoxins; Pick Disease of the Brain; RNA Splicing; Recombinant Fusion Proteins; Two-Hybrid System Techniques; tau Proteins
Saitohin is a gene unique to humans and their closest relatives, the function of which is not yet known. Saitohin contains a single polymorphism (Q7R), and its Q and R alleles belong to the H1 and H2 tau haplotype, respectively. The Saitohin Q allele confers susceptibility to several neurodegenerative diseases. To get a handle on Saitohin function, we used it as a bait in a yeast two-hybrid screen. By this assay and subsequent co-immunoprecipitation and glutathione S-transferase pull-down assays, we discovered and confirmed that Saitohin interacts with peroxiredoxin 6, a unique member of that family that is bifunctional and the levels of which increase in Pick disease. The strength of the interaction appeared to be allele-specific, giving the first distinction between the two forms of Saitohin.
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Citation: J Biol Chem. 2005 Nov 25;280(47):39268-72. Epub 2005 Sep 26. Link to article on publisher's site