DARPP-32 binds to tra2-beta1 and influences alternative splicing
Department of Cell Biology
Medical Subject Headings
*Alternative Splicing; Animals; Cell Line; Cells, Cultured; Dopamine and cAMP-Regulated Phosphoprotein 32; inhibitors; Exons; Humans; Models, Biological; Nerve Tissue Proteins; Neurons; Protein Binding; Protein Phosphatase 1; RNA Precursors; RNA Processing, Post-Transcriptional; RNA, Small Interfering; RNA-Binding Proteins; Rats; Signal Transduction
The majority of human genes undergo alternative splicing, which is frequently altered in response to physiological stimuli. DARPP-32 (dopamine and cAMP regulated phosphoprotein, 32kDa) is a component of PKA-dependent signaling pathways. Here we show that DARPP-32 binds directly to the splicing factor tra2-beta1 (transformer 2). DARPP-32 changes the usage of tra2-beta1 dependent alternative exons in a concentration-dependent manner, suggesting that the DARPP-32:tra2-beta1 interaction is a molecular link between signaling pathways and pre-mRNA processing.
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Citation: Biochim Biophys Acta. 2010 May-Jun;1799(5-6):448-53. Epub 2010 Jan 13. Link to article on publisher's site